sequence design to enhance Fc / Fc-g Receptor binding

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    • May 23, 2017 at 4:00 pm #2659
      Anonymous

        Dear all,

        I recently read a paper from Xencor describing their excellent work on mutation design of antibody Fc regions to increase the binding affinity to Fc-gamma receptor III-A and consequently enhance the Fc effector function.  In the original paper’s supporting text of method (http://www.pnas.org/content/suppl/2006/03/02/0508123103.DC1/08123SuppText.pdf), it mentioned a computation method called PDA which was invented by Dr. Mayo in 1996. Since Mayo was cofounder of MSI and later Accelrys, so I guess this method may become part of Accelrys discovery studio (I am not sure).

        since Rosetta is versatile modeling suite, I am trying to use Rosetta to repeat the whole design process of that paper. I think RosettaDesign could be the starting point, however I also think a customized RosettaXML script could be fit for the purpose of iterative design cycles.

        I am wondering if someone is also interested in using Rosetta to do this design. I need some enlightments from your expertise. Thanks.

        regards,

        Jarod

         

      • June 1, 2017 at 5:00 pm #12358
        Anonymous

          There is currently ongoing work on antibody design, but mainly in the Fv, primarily in the CDRs (in preparation).  I could certainly help with constructing an XML or PyRosetta script to do the type of design that you are after – but I don’t think there is any extremely specific design algorithm implemented to handle this type of thing. 

          -Jared

        • June 1, 2017 at 5:00 pm #12879
          Anonymous

            There is currently ongoing work on antibody design, but mainly in the Fv, primarily in the CDRs (in preparation).  I could certainly help with constructing an XML or PyRosetta script to do the type of design that you are after – but I don’t think there is any extremely specific design algorithm implemented to handle this type of thing. 

            -Jared

          • June 1, 2017 at 5:00 pm #13400
            Anonymous

              There is currently ongoing work on antibody design, but mainly in the Fv, primarily in the CDRs (in preparation).  I could certainly help with constructing an XML or PyRosetta script to do the type of design that you are after – but I don’t think there is any extremely specific design algorithm implemented to handle this type of thing. 

              -Jared

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