error: Unable to open weights/patch file in using ddg_monomer.linuxgccrelease

Dear Friends and experts

I am trying to use “ddg_monomer.linuxgccrelease”. First I want to pre-minimize the structure and get mincst.log file. I put all options in a flag file called pre_minimize

I run the following command:

/home/jy/rosetta3.10/rosetta_bin_linux_2018.33.60351_bundle/main/source/bin/minimize_with_cst.linuxgccrelease @pre_minimize

1. To pre-minimize, the command from protocol is as following:

/path/to/minimize_with_cst.linuxgccrelease -in:file:l lst -in:file:fullatom -ignore_unrecognized_res -fa_max_dis 9.0 -database /path/to/rosetta_database/ -ddg::harmonic_ca_tether 0.5 -score:weights standard -ddg::constraint_weight 1.0 -ddg::out_pdb_prefix min_cst_0.5 -ddg::sc_min_only false -score:patch rosetta_database/scoring/weights/score12.wts_patch > mincst.log

the content of pre_minimize flag file is what follows

-in:file:s adtppvg_10.pdb

-in:file:fullatom

-ignore_unrecognized_res

-fa_max_dis 9.0

-database /home/jy/rosetta3.10/rosetta_bin_linux_2018.33.60351_bundle/main/database

-ddg::harmonic_ca_tether 0.5

-score:weights pre_talaris_2013_standard

-restore_pre_talaris_2013_behavior

-ddg::constraint_weight 1.0

-ddg::out_pdb_prefix min_cst_0.5

-ddg::sc_min_only false

-score:patch score12.wts_patch > mincst.log

I changed -score:wights standard to score12, ref2015 and re_talaris_2013_standard, respectively. Then I run the command, all of which returned me same error message as follows:

ERROR: Unable to open weights/patch file. None of (./)> or (./)>.wts_patch or /home/jy/rosetta3.10/rosetta_bin_linux_2018.33.60351_bundle/main/database/scoring/weights/> or /home/jy/rosetta3.10/rosetta_bin_linux_2018.33.60351_bundle/main/database/scoring/weights/>.wts_patch exist

ERROR:: Exit from: src/core/scoring/ScoreFunction.cc line: 2749

I aslo tried copy weights files to current working directory  or specify the full path to weights file which is /home/jinyan/rosetta3.10/rosetta_bin_linux_2018.33.60351_bundle/main/database/scoring/weights/pre_talaris_2013_standard. But I still get same error message. I do not know how to ssolve this problem and I am wondering whether you could please help me out. I would be much appreciated.

Thanks again

Best regards

xinmiao