ROSETTA design affinity optimization

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    • February 3, 2020 at 9:42 am #3338
      Anonymous

        Hello

        I’m trying to to use rosetta design (http://rosettadesign.med.unc.edu) to increase binding affinity between 2 proteins in 1 pdb file. 

        I want 2 residue ranges to be mutated in chain B but all other residues to remain the same. Therefore I have used this resfile:

        NATRO

        start

        71 – 88 B ALLAAxc EX1

        50 – 56 B ALLAAxc EX1

         

        However, the server mutates residues in both chains. How do I force it to only mutate chain B?

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