Help with filtering (Rosetta scripts)

Hello

I’m running running a pilot ROSETTA script to get the GreedyOptMutation mover with the ddg filter to work. But I get this error message;

ERROR: ERROR: No acceptable mutations found. All possible mutations failed at least one filter!

Any ideas on how to get the filter to allow trajectories with mutations that improve ddG and increases affinity between the 2 proteins contained in the .pdb?

My .xml looks like this:

<ROSETTASCRIPTS>

   <SCOREFXNS>

       <ScoreFunction name=”r15″ weights=”ref2015″/>

   </SCOREFXNS>

   <RESIDUE_SELECTORS>

   </RESIDUE_SELECTORS>

   <TASKOPERATIONS>

              <ReadResfile name=”res_mutate” filename=”resfile.txt”/>   

   </TASKOPERATIONS>

   <FILTERS>

       <Ddg name=”ddgFilter” scorefxn=”r15″ repack=”1″ chain_num=”2″/>

   </FILTERS>

   <MOVERS>

       <MinMover name=”min_torsion” scorefxn=”r15″ chi=”true” bb=”true” cartesian=”F” /> 

       <GreedyOptMutationMover name=”gopt” task_operations=”res_mutate” filter=”ddgFilter” scorefxn=”r15″ relax_mover=”min_torsion” />

    </MOVERS>

   <APPLY_TO_POSE>

   </APPLY_TO_POSE>

   <PROTOCOLS>

       <Add mover=”gopt”/>

   </PROTOCOLS>

   <OUTPUT />

</ROSETTASCRIPTS>

The resfile looks like this:

NATRO 

start

77 – 88 A ALLAAxc 

51 – 56 A ALLAAxc 

23 – 30 A ALLAAxc