The scripts and input files that accompany this demo can be found in the demos/public directory of the Rosetta weekly releases.


This is a short demo showing how you can design your protein with flexible backbone using Rosetta Scripts. The flxbb mover can be given a blueprint file to extrapolate into a movemap; does design, can do some relaxation; converts your pose to alanine before designing.

To run the demo simple type:

($ROSETTA3= path-to-Rosetta/main/source)

$> $ROSETTA3/bin/rosetta_scripts.default.linuxgccrelease @flags

Blueprint format:

    resnum  residue  (ss_struct)(abego) rebuild
    resnum = consecutive (starting from 1) or 0 (to indicate a new residue not in the input.pdb)
    residue = one letter code amino acid (e.g. V for Valine)
    ss_struct = secondary structure, E,L or H. ss_struct and abego are single-letter and have no space between them.
    abego = abego type (ABEGO), use X if any is allowed
    rebuild = R (rebuild this position) or "." (leave as is)


    1   V  LE  R   (position 1, Val, loop, abego type E, rebuild)
    0   V  EX  R   (insert a residue, Val, sheet, any abego, rebuild)
    2   V  EB  .   (position 2, Val, sheet, abego type B, do not rebuild)