Reply To: Flexible-backbone Rosetta design (partial flexibility)

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June 9, 2009 at 10:00 pm #4094
Anonymous

    > Hi All,
    >
    > I have a question regarding flexible-backbone rosettaDesign. I have a protein complex with two chains, and I want to allow the backbone of only ONE chain (the smaller partner) to be flexible. However, although I have “resfile” specified in my run, the backbone of entire complex was moving freely and so the complex got unfolded. Here are the flags included in my run:
    > -design -mvbb -read_all_chains -fa_input -repack -ex1aro -ex2aro_only -resfile
    >
    > Is it not possible to allow PART of the complex backbone to be flexible, or am I doing something wrong?
    >
    > Any input/information will be greatly appreciated
    >
    > Shirley