Member Site Forums Rosetta++ Rosetta++ – General producing good idealized structures Reply To: producing good idealized structures


    there’s two idealize protocols in Rosetta++, one written using pose and one written before pose. In my experience the pose-idealize works much better. I believe you simply have to add ‘-pose’ along with ‘-idealize’.

    I wouldn’t minimize the starting structure before idealizing. Keep in mind that, barring chain-breaks, idealizing is much more difficult at larger chain lengths and 700 is pretty large.