Reply To: Modeling only part of the protein structure using ROSETTA

[Anonymous]

Hello all,

I’m trying to build up just one part of my protein giving to rosetta a pdb file with the rest of the structure that would have to be frozen…..How can I do this using Abinitio folding mode? Do I have to change the code of the Abinitio mode?
Did you manage to do this?

Thanks in advance for help

Cristina

> Hello all,
> We are trying to calculate a protein ensemble using CS-ROSETTA. We have reasonable evidences to believe that only part of the protein (C terminal part) undergoes conformational change at the condition while the rest (N terminal part) remains as a similar structure that we have already known. The protein has 146 aa, we are just wondering is there a way for ROSETTA to “Freeze” part of the protein and only do the calculation on the other part? Thanks in advance for help.
>
> Qiong