Reply To: Fix missing backbone residues

[Anonymous]

Their occupancy column is zero. Check the PDB file format for better descriptors of the columns (from the PDB, not Rosetta). Basically the “0.00” column tells Rosetta that the atom is not present, so Rosetta ignores it. You can just manually change this to a nonzero value to get it to work.

The nonstandard ordering of the backbone atoms (N CA C O is normal) is also unusual, but I’m not sure it’s a problem.