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I solved it by myself, thanks for the open source.
For “general” alignment file format, it is unbelievable simple:
score nnn.nnn
queryID startPos seq
templID startPos seq
queryID startPos seq
templID startPos seq
–# for start another alignment
score nnn.nnn
queryID startPos seq
templID startPos seq
queryID startPos seq
templID startPos seq
I do not know which aligner generates this “general” file format. Then we need another utility to convert any other aligner’s output to this format. It seems Rosetta-CM calculates identities and gaps in its own code, although I think all the aligner will provide such information with the alignment result.
Anyway, thanks to the Rosetta contributors.
len