Member Site › Forums › Rosetta 3 › Rosetta 3 – General › could I add some constrain to the backbone but not completely fix it when relax? › Reply To: could I add some constrain to the backbone but not completely fix it when relax?
Thanks a lot for your kind reply. It would be a challenge for me to write the constrain by myself.
I would like to add some constrain to the alpha carbon(the position won’t be changed a lot and on the other hand it could still let rosetta to refine other part of the protein) so that it could keep the structure much more like my initial homology model. Otherwise, the refined structure would change a lot after refinement which is not reliabe to some extent, especially the sidechain conformations.
If possilbe, would you please provide me some ready to used scrips? I would be very appreciated for this.