Reply To: how to use rosettadesign to do mutations on protein structure

[bpierce]

> Dear all:
>
> I am learning to use rosettadesign to do mutations on protein structure. I do not know how to prepare the resfile. In my protein, I only need to do two mutations, for examle, V42M and M24V. In such kind of case , how the resfile looks like?
>
> I prepared one like following:
>
> ALLAA
> start
> 24 A NOTAA V
> 42 B NOTAA M
>
> could that make sense? Thanks a lot.
>
>