Actually, if you only want to make a double mutant, this is what you want in your resfile:
NATAA
start
24 A PIKAA M
43 B PIKAA V
The default NATAA will allow non-mutated residues to repack around your mutations, whereas the default ALLAA would have caused all residues everywhere to be designed, resulting in many mutations (not just the 2 you want).
Also, are you working on a dimer? If not, you probably don’t want 2 different chain IDs (A & 
in your resfile. If it’s a dimer and the mutations are on different subunits, you are fine. Also, just a little tip. You might want to specify NATRO as the default, and then use NATAA only for specific residues near your mutations (I get better results that way).
V.
> Dear all:
>
> I am learning to use rosettadesign to do mutations on protein structure. I do not know how to prepare the resfile. In my protein, I only need to do two mutations, for examle, V42M and M24V. In such kind of case , how the resfile looks like?
>
> I prepared one like following:
>
> ALLAA
> start
> 24 A NOTAA V
> 42 B NOTAA M
>
> could that make sense? Thanks a lot.
>
>