Reply To: first run of abinitio in rosetta 3.2 produces an error right away

[Anonymous]

I don’t have a clue what it does either. I’d just skip it. If you find yourself with a psipred_2 file later doing your actual modeling, you can always try it then! You may collect one as a side effect of generating fragments.

Psipred_2 is a secondary structure prediction algorithm; I’m guessing the file contains hints telling Rosetta where beta-hairpins are likely to occur.