Member Site Forums Rosetta 3 Rosetta 3 – Applications demo of molecular replacement with rosetta (mr_rosetta) Reply To: demo of molecular replacement with rosetta (mr_rosetta)


    Reposting for reference:

    For previous blind cases, I rescored the top 10 percent (200 models) with phaser. At that point, if the correct MR solution is among the 200, there should be a very clear score separation … in other words, the top-scoring models would all look very similar (and be in the same orientation). If they do not, then the correct MR solution was probably not in the input set (alternatively, there may be errors in the sequence alignment). In cases where the top models are in agreement, I would generally take 5 or fewer models through the additional relax (often just 1).

    To build the density map phased the model, I used the phenix.maps program ( You might also want to take a look at the phenix interface to mr_rosetta,