Member Site › Forums › Rosetta 3 › Rosetta 3 – General › How to relax only one domain of a protein and keep the rest of them fixed › Reply To: How to relax only one domain of a protein and keep the rest of them fixed
-constrain_relax_segments flag takes a loop definition file as an input, thus I suppose it relaxes the specified loop only. I suspect this is what jtmacd was trying to do with PyRosetta. I’m currently testing the -fix_residues_to_native flag.
I have the notion that relaxing a loop is different from relaxing a whole domain. I have tried to selectively relax my missing domain using the loop relax protocol invoked by this GUI:
but all the secondary structure of the domain disappeared.