Member Site › Forums › Rosetta++ › Rosetta++ – General › Can Rosetta Antibody build a good model for a new sequence?? › Reply To: Can Rosetta Antibody build a good model for a new sequence??
I am sure that RosettaAntibody can predict sequences for which structures do not exist in the PDB. It is difficult to imagine that the 1500 jobs that the server has executed are for sequences which already have a structure in the PDB! I suspect that there is an issue with the sequence that has been used as an input. Probably it does not have one of the key signatures responsible for detecting the start/stop of CDR regions -resulting in a distorted grafted model (FR02.pdb). I recommend that you test you sequence for antibody specific signatures by submitting them to the Abnum server: http://www.bioinf.org.uk/abs/abnum/