Reply To: Modeling phosphate ion binding site in protein.

[Anonymous]

Thanks for the suggestion…

There are crystal structures of proteins with phosphate… I didn’t understand about running protocol runs.. Do I have to dock the crystal structure with phosphate again to look for the binding affinities…

Also, I want to graft the metal binding sites and then I have to check for the binding site affinity for the grafted region. Can rosetta be used in such a way that it can automatically change the amino acid in the grafted region to increase the binding affinity..