Member Site › Forums › Rosetta 3 › Rosetta 3 – Applications › demo of molecular replacement with rosetta (mr_rosetta) › Reply To: demo of molecular replacement with rosetta (mr_rosetta)
June 8, 2011 at 7:38 pm
#5739
Anonymous
I replied to the user directly, but I am reposting the solution below:
The LLG dropping indicates something is going wrong. I think the problem is the sequence identity you give to phaser. Even after refinement in Rosetta, you should keep this value at its original sequence identity (25% or whatever). Using this same value, you should see an increase in LLG.