Reply To: Can Fold-and-dock protocol applied to membrane proteins

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July 25, 2011 at 8:41 am #5902
Anonymous

    No one knows the solution?

    I have tried the following options:
    -database /home/clzhang/rosetta3.2/rosetta_database

    -in:file:fasta
    -in:file:frag3
    -in:file:frag9
    -in:file:spanfile
    -in:file:lipofile
    -symmetry_definition symm_def_dimer.dat
    -symmetry:initialize_rigid_body_dofs

    -abinitio:membrane
    -membrane:no_interpolate_Mpair
    -membrane:Menv_penalties

    But I got complains like: “core.scoring.SymmetricScoreFunction: Warning!!! Using a symmetric score function on a non-symmetric pose”

    Does anyone knows what the problem is?
    Or, currently, symmetry file cannot be recognized by membrane_denovo executable?

    -Justin