antibody protocol fails

[Anonymous]

Hello,

I’m using antibody protocl to graft some models but it fails with the following error:

File: src/protocols/antibody/grafting/chothia_numberer.cc:152

ERROR: Unxpected length of light-fr3 [length=31], length expected to be: [32, 33, 34]!

Do you have any comments on this? I have three sequences (three different antibodies with light and heavy chains) and it works on one of them but the same error on the two others.

Thanks a lot in advance,

Best

[Anonymous]

The framework 3 region in the light chain is an illegal length – 31 instead of 32-34.  Rosetta only has templates and tools for sufficiently canonical antibodies.  It detects your sequence as too noncanonical for it to work with.

[Author]

Posts