connecting pdb files

[Anonymous]

Hi

I has been using RosettaRemodel -remodel:domainFusion:insert_segment_from_pdb for connecting pdb files such as connecting 1 helix pdb file and another 1 helix pdb file.

But it doesnt always work, is there another way to connecting two helices in different pdb file by loop? 

Im wondering what protocols can be used for connecting two pdb files with loop to generate 1 pdb file 

please help me 

Thanks

 

[Anonymous]

I have had good results with

https://new.rosettacommons.org/docs/wiki/scripting_documentation/RosettaScripts/xsd/mover_ConnectChainsMover_type

You have to load both helixes into one pose as seperate chains and then specify which chains to connect to.

 

Best,

Ajasja

[Author]

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