Creating fragment files for proteins less than 27 aa in length

Member Site Forums Rosetta 3 Rosetta 3 – Applications Creating fragment files for proteins less than 27 aa in length

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      Anonymous

        Good day.

        I’d like to make abinitio structure prediction using rosetta of some small proteins (e.g. 2jof, which length is 20 amino acids). 

        Such prediction requires fragment files, which can be generated using web Robetta Server or by youself using PSI-BLAST.

        However, when i tried to submit a job on Robetta there was an error:

        Sequence length must be between 27 and 1000 residues

        Similar restriction was described in rosetta manual dedicated fragment-files:

        Obtain a fasta file for the desired sequence. This file must have 60 characters/line with no white space. Therefore, no less than 60 aa. 

        So, i’d like to ask if there is some way to generate fragment files for proteins, which length less than 27, in order to predict their structure using Rosetta Abinitio?

        Many thanks in advance.

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