De novo structure building with medium resolution map

[Anonymous]

Dear all,

I am trying to build a model for my protein and I have a medium resolution map (~5-6 A). I tried backbone tracing manually, but many regions are dubious. This protein does not have any homologous protein either, so I am looking for tools to do denovo model building with guided electron density. Is there any available protocol to achieve this?

Best,

Buddy

[Anonymous]

Hello Buddy,

As with most things in cryo-EM it’s hard to say without seeing the map. however you’re possibly in the range of our capabilities.

you could try the de-novo protocols we have described at https://dimaiolab.ipd.uw.edu/software/

if that doesn’t work, you can email me at danpf@uw.edu or Dr. DiMaio and we could discuss this further.

Dan

[Anonymous]

Thank you! I will try it!

Buddy

[Author]

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