Error using ddg_monomer application (Assertion `pose.residue(resnum).name1() == wt` failed)

Member Site › Forums › Rosetta 3 › Rosetta 3 – General › Error using ddg_monomer application (Assertion `pose.residue(resnum).name1() == wt` failed)

Viewing 2 reply threads
  • Author
    Posts
    • June 24, 2021 at 9:47 pm #3797
      Anonymous

        Hi, I’ve unsuccessfully tried to run ddg_monomer app. I want to study the estability of a small protein after some mutations. I have used a pre-minimized pdb file as an input file, but I have the following error (last lines):

        apps.public.ddg.ddg_monomer: reading in mutfile

        apps.public.ddg.ddg_monomer: wt is N resnum is 25 and mut is P

        apps.public.ddg.ddg_monomer: wt is Y resnum is 60 and mut is H

        ERROR: Assertion `pose.residue(resnum).name1() == wt` failed.

        ERROR:: Exit from: src/apps/public/ddg/ddg_monomer.cc line: 171

         

        I have found a similar error in the Rosetta forum, but none of the solutions (https://www.rosettacommons.org/content/ddgmonomer-problem, https://www.rosettacommons.org/node/3443) worked for my problem. I am pretty new using Rosetta, so it’s quite possible that I’m doing something wrong.

        I have attached the files I used with the app. The original pdb file has an unsolved loop, and also has a n-glycated residue. I’m not sure if this can be a problem for ddg_monomer.   

        Any help/suggestion is very welcome!

         

          

      • June 25, 2021 at 5:18 pm #15947
        Anonymous

          I’ve also tried dd_monomer with a deglycated and complete chain of my protein without success. 

        • June 25, 2021 at 6:26 pm #15948
          Anonymous

            Sorry, it was my mistake, I didn’t realize that sequence numbering wasn’t consecutive!!

        • Author
          Posts
        Viewing 2 reply threads
        • You must be logged in to reply to this topic.