Hi,
I’ve been recently using rosetta to refine some atomic models into helical cryo-EM maps. Frank DiMaio’s tutorial is really great, but unfortunately it does not cover helical complexes. So far, I’ve been mostly working with symmetry files as they come from make_symmdef_file.pl, as I have a pretty accurate idea of how the complex looks like, and the results are rather nice. However, in the tutorials it is recommended to modify the symmetry files given that the protein is being fit into the density. I’m still a bit confused with the syntax of these symmetry definitions, but if I understand it correctly, it should be something like:
Original:
…
set_dof JUMP_0_0_0 z(27.4284032299595) angle_z
set_dof JUMP_0_0_0_to_com x(15.6928354835077)
set_dof JUMP_0_0_0_to_subunit angle_x angle_y angle_z
…
Modified:
…
set_dof JUMP_0_0_0 z(27.4284032299595) angle_z
set_dof JUMP_0_0_0_to_com x y z
set_dof JUMP_0_0_0_to_subunit angle_x angle_y angle_z
….
I’d really appreciate any input on this.
Regards
Felipe
.
