how to know the precedure of solving a actural bio problem? like interface design?

[Anonymous]

hi 

im a doctorial student in the learning process of rosetta scripts

and i was to solve a problem like i got to design a protein complex A0_B0 with known structure and known complex of A1_B1 A2_B2 all Bi are homologs

i need to design a binder A0 that binds to B0

but I just dont know how

and a teacher told me that i needed to manually docking B0 to like a1

and to use fastdesign,fastrelax, to use taskops of layerdesign,limitaromachi2,PruneBuriedUnsats,and filters like BuriedUnsatHbonds

so how am i supossed to know which mover and taskop to use if i am learning by materials and all myself

is that just experience or ?

what if i am to design a denovo binder or to do structure prediction

what combinations of movers and taskops then? ( assume i know all the meaning of single ones)

[Anonymous]

This task is not easy even for someone with years of Rosetta experience.  I would go onto the wiki and start reading. There is documentation for everything you talked about. Go through the referenced papers, tutorials, etc.  

[Anonymous]

thank you for reply

do you mean referenced papers by those who solved a actural problem or those by bakerlab about how some movers or taskops are built?

 

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