Rosetta Antibody cuts off light chain?

[Anonymous]

Hi all,

I am trying to use Rosetta antibody to predict the structure of the protein 5i19. Here is the input fasta:

>heavy

EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYL QMNSLRAEDTAVYYCAKYDGIYGELDFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGAL TSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS

>light

DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQ
PEDFATYYCQQSYSTPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVT
EQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

 

For some reason, the template modeling process cuts off the light chain at position 109. Any thoughts?

Thank you!

Ziheng Wang

 

[Anonymous]

Dear Ziheng,

RosettaAntibody only models the variable region of the antibody, for the light chain in your case this would be residues 1-109 (under the Chothia numbering convention). We do not consider the sebsequent residues as they comprise the constant domain, which tends to be well-conserved and not involved in antigen binding. However, if the constant region of this antibody is important for your modeling task, I’d be happy to help you add it to your model (it would involve something like modeling the variable domain alone, then finding a structural template for the constant domain, aligning the variable domain to the template, altering the template sequence in the constant domain to match the target, and remodeling the linker regions to connect the modeled variable and constant domains).

Best,

Jeliazko 

[Author]

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