ROSETTA design affinity optimization

[Anonymous]

Hello

I’m trying to to use rosetta design (http://rosettadesign.med.unc.edu) to increase binding affinity between 2 proteins in 1 pdb file. 

I want 2 residue ranges to be mutated in chain B but all other residues to remain the same. Therefore I have used this resfile:

NATRO

start

71 – 88 B ALLAAxc EX1

50 – 56 B ALLAAxc EX1

 

However, the server mutates residues in both chains. How do I force it to only mutate chain B?

[Author]

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