Reply To: errors while running rosetta 3.1

[Anonymous]

I’ve never used CSrosetta, but both errors sound to me like you have a number-of-residues mismatch somewhere in your process. For example, you want to predict the structure of a 90 residue protein, but you generated fragments using the FASTA for only 85 residues’ worth of protein. Rosetta is not smart enough to silently add things like 6xHis-tags to the ends of proteins (or remove them). That’s the first place I’d look for a solution.