generating 10 lowest energy structures from abinitio

Member Site Forums Rosetta 3 Rosetta 3 – General generating 10 lowest energy structures from abinitio

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    • #577

        I ran the abinitio protocol for a protein with its fasta sequence only. I generated the fragments using the Robetta server. I got only 1 pdb file as an output. How do I get it to generate 10 lowest energy structures in pdb format?

      • #4448

          did you try -nstruct 10?

        • #4457

            Dear Rosetta devs and community,

            What does Robetta use for decoy selection, and is this method codified in any protocols in the Rosetta3.1 source? I’ve generated a large set of structure decoys using AbinitioRelax, and I’d like to select the top 5 decoys. I can attempt to reproduce the clustering method described in Bonneau et al {JMB (2002) 322:65-78}, but is this the latest and greatest approach?

            Thanks much,

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