Member Site › Forums › Rosetta 3 › Rosetta 3 – General › Homology modeling
Is there good documentation or a tutorial on how to use Rosetta 3.0 to construct a homology model based on a user-input template?
Documentation is not clear (or is absent).
Any help is most welcomed. I am able to generate a backbone model, but unable to generate a fullatom model.
The error I am getting when running the following:
-loops::frag_sizes 9 3
-loops::frag_files aaquery09_05.200_v1_3 aaquery03_05.200_v1_3
“ERROR: Could not find disulfide partner for residue”
Do you have disulfides in your protein?
As of 3.0, the “detect_disulfides” flag defaults to true, you can try turning this to false, especially if you do not have disulfides.
There is a flag “fix_disulf” which lets you specify where you know the disulfides are – I’ve never used it and have no idea if it’s compatible with loop modeling.